KMID : 1007520200290081141
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Food Science and Biotechnology 2020 Volume.29 No. 8 p.1141 ~ p.1148
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Evaluation of 3M¢â loop-mediated isothermal amplification-based kit and 3M¢â ready-to-use plating system for detection of Listeria in naturally contaminated leafy vegetables, chicken, and their related processing environments
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Abatcha Mustapha Goni
Tan Pei Ling Chuah Li-Oon Rusul Gulam Chandraprasad S. R. Effarizah Mohd Esah
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Abstract
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The effectiveness of two different rapid methods involving the 3M¢â molecular detection assay Listeria and the 3M¢â Petrifilm environmental Listeria Plate were evaluated for the rapid detection of Listeria from naturally contaminated vegetables and chicken-processing environments against the standard culture-based method. A total of 178 samples were examined for the presence of Listeria. A total of 47/178 (26.4%) by standard ISO culture-based method (EN ISO 11290-1), 42/178 (23.6%) by 3M¢â MDA Listeria and 40/178 (22.5%) by 3M¢â Petrifilm EL Plate showed positive results, respectively. The accuracy, sensitivity, specificity, positive predictive value, and negative predictive value for 3M¢â MDA Listeria and 3M¢â Petrifilm EL Plate were 97.2, 89.4, 99.3, 97.7, 96.4% and 96.1, 85.1, 100.0, 100.0, 94.9%, respectively. Based on the Cohen¡¯s Kappa value, there was a complete and robust concordance between 3M¢â MDA Listeria (0.911) and 3M¢â Petrifilm EL Plates (0.894) as compared to the standard culture-based method.
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KEYWORD
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3M¢â MDA listeria, 3M¢â petrifilm EL plate, Listeria
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